other:Article Title: CDCP1 is a novel marker of the most aggressive human triple-negative breast cancers
Article Snippet: In the biochemical analyses, we used rabbit polyclonal antibody against CDCP1 (Merck Millipore); rabbit polyclonal phospho-CDCP1 (Tyr734) (Cell Signaling); mouse monoclonal anti-Src, clone GD11 (Merck Millipore); rabbit polyclonal phospho-Src family (Tyr416) (Cell Signaling); rabbit polyclonal anti-PKCδ (Cell Signaling); rabbit polyclonal phospho-PKCδ (Tyr311) (Cell Signaling); mouse monoclonal anti-vinculin, clone hVIN-1 (Sigma); anti-rabbit or -mouse IgG (GE Healthcare) as the secondary antibody; and peroxidase-linked mouse monoclonal anti-actin (Sigma-Aldrich).
Article Title: Helicobacter Pylori Targets the EPHA2 Receptor Tyrosine Kinase in Gastric Cells Modulating Key Cellular Functions.
Article Snippet: The antibodies used in this study included rabbit polyclonal anti-AKT (#9272; Cell Signaling Technologies Inc., Danvers, MA, USA), rabbit polyclonal anti-phospho-Ser473-AKT (#4060; Cell Signaling Technology), rabbit polyclonal anti-EPHA2 (clone C-20; sc-924; Santa Cruz Biotechnology Inc., Dallas, TX, USA), rabbit monoclonal anti-EPHA2 (clone D4A2; #6997; Cell Signaling Technology), rabbit mAb phosphoSer897-EPHA2 (Clone D9A1; #6347; Cell Signaling Technology), rabbit pAb phospho Tyr772-EPHA2 (#8244; Cell Signaling Technology), mouse monoclonal anti-GAPDH (clone 0411; sc-47724; Santa Cruz), mouse monoclonal anti-alpha 1 integrin (clone SR84; #559594, BD Biosciences, San Jose, CA, USA), mouse monoclonal anti-beta 1 integrin (clone JB1B; sc-59829, Santa Cruz Biotechnology), rabbit polyclonal anti-p44/42 MAPK (ERK1/2; clone 137F5; #4695; Cell Signaling Technology), rabbit monoclonal anti-phospho Thr202/Tyr204-p44/42 MAPK (ERK1/2; clone D13.14.4E; #4370; Cell Signaling Technology), Alexa Fluor 488 goat anti-rabbit IgG (#A11034; Thermo Fisher Scientific, Waltham, MA, USA), rabbit polyclonal IgG (ab27478, Abcam, Cambridge, UK), mouse monoclonal antibody PY99 (sc-7020; Santa Cruz Biotechnology), mouse monoclonal anti-SRC (clone L4A1; #2110; Cell Signaling Technology), rabbit polyclonal anti-phospho Tyr416-SRC Family (#2101; Cell Signaling Technology), and mouse monoclonal anti-α-Tubulin (clone B-5-1-2; #T5168; Sigma-Aldrich Co., St. Louis, MO, USA).
Article Title: Helicobacter Pylori Targets the EPHA2 Receptor Tyrosine Kinase in Gastric Cells Modulating Key Cellular Functions
Article Snippet: The antibodies used in this study included rabbit polyclonal anti-AKT (#9272; Cell Signaling Technologies Inc., Danvers, MA, USA), rabbit polyclonal anti-phospho-Ser473-AKT (#4060; Cell Signaling Technology), rabbit polyclonal anti-EPHA2 (clone C-20; sc-924; Santa Cruz Biotechnology Inc., Dallas, TX, USA), rabbit monoclonal anti-EPHA2 (clone D4A2; #6997; Cell Signaling Technology), rabbit mAb phosphoSer897-EPHA2 (Clone D9A1; #6347; Cell Signaling Technology), rabbit pAb phospho Tyr772-EPHA2 (#8244; Cell Signaling Technology), mouse monoclonal anti-GAPDH (clone 0411; sc-47724; Santa Cruz), mouse monoclonal anti-alpha 1 integrin (clone SR84; #559594, BD Biosciences, San Jose, CA, USA), mouse monoclonal anti-beta 1 integrin (clone JB1B; sc-59829, Santa Cruz Biotechnology), rabbit polyclonal anti-p44/42 MAPK (ERK1/2; clone 137F5; #4695; Cell Signaling Technology), rabbit monoclonal anti-phospho Thr202/Tyr204-p44/42 MAPK (ERK1/2; clone D13.14.4E; #4370; Cell Signaling Technology), Alexa Fluor 488 goat anti-rabbit IgG (#A11034; Thermo Fisher Scientific, Waltham, MA, USA), rabbit polyclonal IgG (ab27478, Abcam, Cambridge, UK), mouse monoclonal antibody PY99 (sc-7020; Santa Cruz Biotechnology), mouse monoclonal anti-SRC (clone L4A1; #2110; Cell Signaling Technology), rabbit polyclonal anti-phospho Tyr416-SRC Family (#2101; Cell Signaling Technology), and mouse monoclonal anti-α-Tubulin (clone B-5-1-2; #T5168; Sigma-Aldrich Co., St. Louis, MO, USA).
Western Blot:Article Title: Neuropilin-1 mediates vascular permeability independently of vascular endothelial growth factor receptor-2 activation.
Article Snippet: The mouse monoclonal VioBright FITC anti-NRP1 antibody (Miltenyi) and a mouse isotype control (Abcam) were used for flow cytometry. .. For Western blots, the rabbit monoclonal anti–VEGFR-2, mouse monoclonal anti–phospho-p44/42 mitogen-activated protein kinase (MAPK) Thr202/Tyr204, rabbit monoclonal anti–phospho-Akt Ser473, rabbit polyclonal anti-Akt, rabbit monoclonal anti–phospho-p38 Thr180/Tyr182, rabbit monoclonal anti-p38, rabbit polyclonal anti–phospho-Src Tyr416, mouse monoclonal anti-Src, and rabbit monoclonal anti–phospho-FAK Tyr397 were purchased from Cell Signaling Technology. .. Rabbit polyclonal anti-ERK1/2, rabbit polyclonal anti-FAK, goat polyclonal anti-NRP1, goat polyclonal anti-actin, and mouse monoclonal anti-GAPDH were from Santa Cruz Biotechnology.
Article Title: FK506-binding protein 10 (FKBP10) regulates lung fibroblast migration via collagen VI synthesis
Article Snippet: Phosphorylated SRC Proto-Oncogene, Non-Receptor Tyrosine Kinase , p-Src , rabbit polyclonal anti-pSrc (Tyr416) , Cell Signaling, Boston, USA , WB. .. SRC Proto-Oncogene, Non-Receptor Tyrosine Kinase , Src , mouse monoclonal anti-Src , Cell Signaling, Boston, USA , WB. .. Talin 1 , TLN1 , mouse monoclonal anti-TLN1 , Sigma Aldrich, Dt. Louis, USA , WB, IF, PLA.
Incubation:Article Title: Angiopoietin-Like 4 Attenuates Brain Edema and Neurological Deficits in a Mouse Model of Experimental Intracerebral Hemorrhage
Article Snippet: Protein samples (20 μg per lane) were separated by 10% SDS-PAGE and transferred to polyvinylidene difluoride membranes, then incubated with 3% BSA (catalogue no. G5001; Servicebio Technology Co., LTD, Wuhan, China) for 2 h at room temperature. .. Four primary antibodies were incubated with the membranes overnight at 4°C: rabbit monoclonal anti-VE-cadherin (1: 1000), mouse monoclonal anti-Claudin-5 (1: 1000), mouse monoclonal anti-Src (catalogue no. 2115, dilution, 1: 1000; Cell Signaling Technology, Danvers, MA, USA.), and rabbit monoclonal anti-phospho-Src (catalogue no. 2101, dilution, 1: 1000; Cell Signaling Technology, Danvers, MA, USA). .. Membranes were washed 6 times ×5 min with PBS and subsequently incubated with the following secondary antibodies: goat anti-rabbit horseradish peroxidase-conjugated IgG (catalogue no. AB501, dilution, 1: 3000; Novoprotein, Shanghai, China) and goat anti-mouse horseradish peroxidase-conjugated IgG (catalogue no. GB23301, dilution, 1: 2500; Servicebio Technology Co., LTD, Wuhan, China).
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